Protein_Domain

Part:BBa_K1390014:Design

Designed by: Stefan Dübel   Group: iGEM14_Braunschweig   (2014-07-04)


mmoC-His


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 486
    Illegal NgoMIV site found at 664
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 625
    Illegal BsaI site found at 690
    Illegal BsaI.rc site found at 733


Design Notes

the brick is constructed in the RCF10 standard


Source

This brick was amplified with primers adding a His6-tag to the brick BBa_K1390002

References